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pentr express donor vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pentr express donor vector
    Pentr Express Donor Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+expression+vector/bio_rxiv__2023__04__19__537538-204-7-17
    Average 86 stars, based on 1 article reviews
    pentr express donor vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Functional Interaction between Pregnane X Receptor and Yes-Associated Protein in Xenobiotic-Dependent Liver Hypertrophy and Drug Metabolism.
    Article Snippet: .. The open reading frames of mYAP and hYAP were isolated from each expression plasmid (described previously) by PCR using specific primer sets (Table 1) and subcloned into the appropriate plasmids with the ViraPower Adenoviral Expression System, pENTR Directional TOPO Cloning, and pAd/CMV/V5-DEST Gateway Vector kits (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. One day before transfection, 293T cells were seeded in 6-well plates (Sanplatec, Osaka, Japan) at 5 105 cells/well, and the plasmids that had been linearized with PacI (New England BioLabs, Ipswich, MA) were transfected into the 293T cells using FuGENE HD (Promega).

    Expressing:

    Article Title: Functional Interaction between Pregnane X Receptor and Yes-Associated Protein in Xenobiotic-Dependent Liver Hypertrophy and Drug Metabolism.
    Article Snippet: .. The open reading frames of mYAP and hYAP were isolated from each expression plasmid (described previously) by PCR using specific primer sets (Table 1) and subcloned into the appropriate plasmids with the ViraPower Adenoviral Expression System, pENTR Directional TOPO Cloning, and pAd/CMV/V5-DEST Gateway Vector kits (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. One day before transfection, 293T cells were seeded in 6-well plates (Sanplatec, Osaka, Japan) at 5 105 cells/well, and the plasmids that had been linearized with PacI (New England BioLabs, Ipswich, MA) were transfected into the 293T cells using FuGENE HD (Promega).

    Article Title: Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa
    Article Snippet: .. Coding sequences of Ptr4CL3, Ptr4CL5, PtrHCT1, and PtrHCT6 without stop-codons were transferred from the pENTR gateway vector into pET101-DEST (C-terminal 6 × His tag) expression vector (K10101, Invitrogen) to generate the pET101:: Gene-6 × His plasmids for recombinant protein expression in Escherichia coli . ..

    Article Title: Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) Screened from the Gut Microbiota of the Common Black Slug ( Arion ater )
    Article Snippet: The PCR-amplified product (654 bp) was cloned into an entry vector pENTR/SD/D/TOPO (ThermoFischer Scientific Leicester, UK) following the manufacturer’s protocol. .. To generate the recombinant expression vector , pENTR: Gene_id_40363 was recombined with the destination vector pDEST42 (ThermoFischer Scientific, Leicester, UK) following the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Functional Interaction between Pregnane X Receptor and Yes-Associated Protein in Xenobiotic-Dependent Liver Hypertrophy and Drug Metabolism.
    Article Snippet: .. The open reading frames of mYAP and hYAP were isolated from each expression plasmid (described previously) by PCR using specific primer sets (Table 1) and subcloned into the appropriate plasmids with the ViraPower Adenoviral Expression System, pENTR Directional TOPO Cloning, and pAd/CMV/V5-DEST Gateway Vector kits (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. One day before transfection, 293T cells were seeded in 6-well plates (Sanplatec, Osaka, Japan) at 5 105 cells/well, and the plasmids that had been linearized with PacI (New England BioLabs, Ipswich, MA) were transfected into the 293T cells using FuGENE HD (Promega).

    Article Title: Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa
    Article Snippet: .. Coding sequences of Ptr4CL3, Ptr4CL5, PtrHCT1, and PtrHCT6 without stop-codons were transferred from the pENTR gateway vector into pET101-DEST (C-terminal 6 × His tag) expression vector (K10101, Invitrogen) to generate the pET101:: Gene-6 × His plasmids for recombinant protein expression in Escherichia coli . ..

    Article Title: Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) Screened from the Gut Microbiota of the Common Black Slug ( Arion ater )
    Article Snippet: The PCR-amplified product (654 bp) was cloned into an entry vector pENTR/SD/D/TOPO (ThermoFischer Scientific Leicester, UK) following the manufacturer’s protocol. .. To generate the recombinant expression vector , pENTR: Gene_id_40363 was recombined with the destination vector pDEST42 (ThermoFischer Scientific, Leicester, UK) following the manufacturer’s protocol. ..

    Polymerase Chain Reaction:

    Article Title: Functional Interaction between Pregnane X Receptor and Yes-Associated Protein in Xenobiotic-Dependent Liver Hypertrophy and Drug Metabolism.
    Article Snippet: .. The open reading frames of mYAP and hYAP were isolated from each expression plasmid (described previously) by PCR using specific primer sets (Table 1) and subcloned into the appropriate plasmids with the ViraPower Adenoviral Expression System, pENTR Directional TOPO Cloning, and pAd/CMV/V5-DEST Gateway Vector kits (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. One day before transfection, 293T cells were seeded in 6-well plates (Sanplatec, Osaka, Japan) at 5 105 cells/well, and the plasmids that had been linearized with PacI (New England BioLabs, Ipswich, MA) were transfected into the 293T cells using FuGENE HD (Promega).

    Cloning:

    Article Title: Functional Interaction between Pregnane X Receptor and Yes-Associated Protein in Xenobiotic-Dependent Liver Hypertrophy and Drug Metabolism.
    Article Snippet: .. The open reading frames of mYAP and hYAP were isolated from each expression plasmid (described previously) by PCR using specific primer sets (Table 1) and subcloned into the appropriate plasmids with the ViraPower Adenoviral Expression System, pENTR Directional TOPO Cloning, and pAd/CMV/V5-DEST Gateway Vector kits (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. One day before transfection, 293T cells were seeded in 6-well plates (Sanplatec, Osaka, Japan) at 5 105 cells/well, and the plasmids that had been linearized with PacI (New England BioLabs, Ipswich, MA) were transfected into the 293T cells using FuGENE HD (Promega).

    other:

    Article Title: The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field‐stimulated osteoblastic differentiation
    Article Snippet: 1Fundamental Medical Science Research Laboratories, The 940th Hospital of Joint Logistic Support Force, People's Liberation Army of China, Lanzhou, P. R. China 2Department of Bioengineering, School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, P. R. China 3Key Laboratory of Stem Cells and Gene Drugs of Gansu Province, Lanzhou, P. R. China 4UniSA Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, Australia

    Article Title: Ethylene and RIPENING INHIBITOR Modulate Expression of SlHSP17.7A, B Class I Small Heat Shock Protein Genes During Tomato Fruit Ripening
    Article Snippet: For overexpression , SlMADS-RIN coding sequence was amplified from breaker stage cDNA and then cloned into the pENTR/D-TOPO donor vector (Invitrogen).

    Article Title: Neuronal microRNAs modulate TREK two-pore domain K + channel expression and current density
    Article Snippet: The murine Trek1 (3080nt) and Trek2 (2362nt) CDS+3ʹUTR PCR products were then amplified using the Phusion polymerase (ThermoFisher) (for PCR conditions and primer sequences see Supplementary Tables 1 and 2) and cloned between the SalI and EcoRV restriction sites of the pENTR-GD expression vector (ThermoFisher).

    Article Title: The calcium sensor TaCBL4 and its interacting protein TaCIPK5 are required for wheat resistance to stripe rust fungus.
    Article Snippet: The coding sequence of TaCBL4 was cloned into pENTR/D-TOPO (Life Technologies, https://www.thermofisher.com/us/en/home.html) using genespecific DNA primers (Table S2), then pENTR/D-TOPO expression construct of TaCBL4 was recombined into pGWB11 vector using LR Clone methods (Invitrogen, Carlsbad, CA, USA) to yield an N-terminal fusion to the flagepitope.

    Article Title: Stomatal Development and Gene Expression in Rice Florets.
    Article Snippet: 1Grantham Centre for Sustainable Futures, University of Sheffield, Sheffield S10 2TN, UK 2School of Biosciences, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK 3International Rice Research Institute, DAPO 7777, Metro Manila, Philippines 4Trait Diversity and Function, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, UK 5Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Mexico City 06600, Mexico

    Recombinant:

    Article Title: Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa
    Article Snippet: .. Coding sequences of Ptr4CL3, Ptr4CL5, PtrHCT1, and PtrHCT6 without stop-codons were transferred from the pENTR gateway vector into pET101-DEST (C-terminal 6 × His tag) expression vector (K10101, Invitrogen) to generate the pET101:: Gene-6 × His plasmids for recombinant protein expression in Escherichia coli . ..

    Article Title: Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) Screened from the Gut Microbiota of the Common Black Slug ( Arion ater )
    Article Snippet: The PCR-amplified product (654 bp) was cloned into an entry vector pENTR/SD/D/TOPO (ThermoFischer Scientific Leicester, UK) following the manufacturer’s protocol. .. To generate the recombinant expression vector , pENTR: Gene_id_40363 was recombined with the destination vector pDEST42 (ThermoFischer Scientific, Leicester, UK) following the manufacturer’s protocol. ..



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    Image Search Results


    (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Journal: bioRxiv

    Article Title: tRNA thiolation defects disrupt cellular proteostasis and tissue homeostasis in mammals

    doi: 10.1101/2025.10.24.684405

    Figure Lengend Snippet: (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Article Snippet: For CTU2 knockdown lines we utilized the GLTR system, an all-in-one lentiviral, doxycycline inducible short hairpin RNA (shRNA) expression vector (Addgene 55790, 58246).

    Techniques: Western Blot, Knockdown, shRNA, Control, Northern Blot, Biomarker Discovery, Staining, Over Expression, Two Tailed Test, Immunofluorescence

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software